| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.219639 |
| Chromosome: | chromosome_3 |
| Location: | 9155687 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g212305 | FAP181 | Flagellar Associated Coiled-Coil Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GACTCCACCCAGCCGCCGCCCAGCCGCGGC |
| Internal bar code: | ATGCAGAGCGGAGCGCGGTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 878 |
| LEAP-Seq percent confirming: | 99.8512 |
| LEAP-Seq n confirming: | 671 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCAGCTACCTCAATGTGC |
| Suggested primer 2: | CATGTGCAAACACCAAGGTC |