| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.219653 |
| Chromosome: | chromosome 16 |
| Location: | 6881053 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676421 | TOC1,PRR1 | Pseudo Response Regulator 1; (1 of 1) PTHR26402//PTHR26402:SF465//PTHR31319 - RESPONSE REGULATOR OF TWO-COMPONENT SYSTEM // TWO-COMPONENT RESPONSE REGULATOR-LIKE APRR1-RELATED // FAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTATTGGCACCTATGCGTGCGCCAGTGAG |
| Internal bar code: | AAGCTGATGGAGGGGAGGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 992 |
| LEAP-Seq percent confirming: | 99.3099 |
| LEAP-Seq n confirming: | 3022 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTCACAGCTGCTACAGGC |
| Suggested primer 2: | CTGTTGGGTTCAGTGGGTCT |