Insertion junction: LMJ.RY0402.219660_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.219660
Chromosome:chromosome 3
Location:7565054
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g204900 (1 of 1) PTHR33602:SF1 - REGULATORY PROTEIN RECX intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGGTGCTCAGGGGGTTCGGACCTCTTGA
Internal bar code:AAGCATCGTTGCGGAATTTCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.926262
LEAP-Seq n confirming:51
LEAP-Seq n nonconfirming:5455
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGTCAAATCACCACATCG
Suggested primer 2:CGCTTTCCACCTGCTTCTAC