| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.219665 |
| Chromosome: | chromosome 14 |
| Location: | 86079 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTGCTCGGCACCTGCCCAGCGCACACG |
| Internal bar code: | AATTGAGTGCTCAACTCACACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 97.4644 |
| LEAP-Seq n confirming: | 6496 |
| LEAP-Seq n nonconfirming: | 169 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTGGGTCATGTTGCACT |
| Suggested primer 2: | GTCTGAGGCAAGTGAAAGGC |