| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219667 |
| Chromosome: | chromosome 11 |
| Location: | 1234766 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467699 | SEC1 | Vesicle trafficking protein Sec1; (1 of 1) K15292 - syntaxin-binding protein 1 (STXBP1, MUNC18-1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGGCGTGCTGGCTGCAGGGGCGAGCAA |
| Internal bar code: | CCCGGGAGCGCACGCAGCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 300 |
| LEAP-Seq percent confirming: | 99.3876 |
| LEAP-Seq n confirming: | 1623 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGGCACATCAACTTTTTG |
| Suggested primer 2: | TACGGCAATTTGACAAACGA |