| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.219672 |
| Chromosome: | chromosome 14 |
| Location: | 1205580 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616050 | (1 of 8) PF00169 - PH domain (PH) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTCAGCTGCTCGGAAAGGATGTCCAGAT |
| Internal bar code: | GTAGCGTCGACCGCCTACGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 77 |
| LEAP-Seq percent confirming: | 98.7654 |
| LEAP-Seq n confirming: | 160 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGTAAACACTCGGCCAT |
| Suggested primer 2: | ATTCACGCCGTACATGGTTT |