| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.219703 |
| Chromosome: | chromosome 8 |
| Location: | 4151928 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379900 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTAGCACAGCGGAAGAGGGTTGACCTGAG |
| Internal bar code: | TACTCTTGGAATTAACATTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 99.32 |
| LEAP-Seq n confirming: | 9348 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTGGTCAGAACCAAAACA |
| Suggested primer 2: | TTTTCCTGTGCTTTCCTGCT |