Insertion junction: LMJ.RY0402.219714_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.219714
Chromosome:chromosome 13
Location:1984379
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g576600 Conserved Protein with Similarity to Catalytic domain of Protein Tyrosine Kinases; (1 of 1) PTHR23257//PTHR23257:SF405 - SERINE-THREONINE PROTEIN KINASE // MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 7-LIKE-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGATAGTCCTGCGCTAGTTCCCACTGAGTC
Internal bar code:CGAAATTCCATAGGTAGCATTC

Confirmation - LEAP-Seq

LEAP-Seq distance:713
LEAP-Seq percent confirming:98.6414
LEAP-Seq n confirming:3485
LEAP-Seq n nonconfirming:48
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCCACTTAAGGCTGTCCA
Suggested primer 2:GGGTGTGAATTTGTCAGGCT