| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.219727 |
| Chromosome: | chromosome 4 |
| Location: | 2835774 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224200 | (1 of 2) PTHR18841//PTHR18841:SF0 - VITELLINE MEMBRANE OUTER LAYER PROTEIN I-RELATED // PROTEIN C08G5.3, ISOFORM A-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAACCACATTTTTTCCTTCCCGCCGCCG |
| Internal bar code: | TTCCAGTACTGTCCTGTCTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1243 |
| LEAP-Seq percent confirming: | 99.64 |
| LEAP-Seq n confirming: | 2214 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGAGGCTCAAGGTGAGG |
| Suggested primer 2: | GGATGTACGATATGTCCGGG |