| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.219738 |
| Chromosome: | chromosome_14 |
| Location: | 913421 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g613600 | HSP70H | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGCTCGGCTCTTGCCTGCCTCTTGTCGGGG |
| Internal bar code: | TAAGGGTAAGTTACGACGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 934 |
| LEAP-Seq percent confirming: | 99.8124 |
| LEAP-Seq n confirming: | 5853 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGTACATACCTCGTGCG |
| Suggested primer 2: | ACCGGCGGTAATAGTAGCCT |