Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.219752 |
Chromosome: | chromosome_12 |
Location: | 9144888 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g542500 | MOC1 | Mitochondrial transcription termination factor | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTTGAACTGAACCCTGTGGTAGTTGAAGGC |
Internal bar code: | GCTAACTGCTGCATGCGAACCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 622 |
LEAP-Seq percent confirming: | 99.6933 |
LEAP-Seq n confirming: | 325 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACACCGTCATCACCTTCCTC |
Suggested primer 2: | CGCCTCACAGTTAGCCTTTC |