| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.219752 |
| Chromosome: | chromosome_13 |
| Location: | 3479063 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g587700 | SDR33 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCGGAAAAGAATTGGCTGGAGCGTTTAGT |
| Internal bar code: | CACCGTTCGGCTTGTGCACTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 882 |
| LEAP-Seq percent confirming: | 99.2764 |
| LEAP-Seq n confirming: | 5351 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTTGCCGTAGAACTGCT |
| Suggested primer 2: | TTGATGAGGCAGCACTTGAC |