| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.219755 |
| Chromosome: | chromosome 17 |
| Location: | 863776 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g702400 | PUS5 | (1 of 1) 3.5.4.26//5.4.99.28 - Diaminohydroxyphosphoribosylaminopyrimidine deaminase // tRNA pseudouridine(32) synthase / Pseudouridine synthase RluA; RNA pseudouridine synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACGCTCGATTGAGACCCCCTTCCCGCTC |
| Internal bar code: | GCTTGACCCCAACGATAGCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 183 |
| LEAP-Seq percent confirming: | 99.5215 |
| LEAP-Seq n confirming: | 416 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTTTGCCTTTCGTTCATC |
| Suggested primer 2: | TTCTTACGGTACTGGCCCAC |