| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219783 |
| Chromosome: | chromosome 13 |
| Location: | 2651635 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g581600 | ASA4 | Mitochondrial F1F0 ATP synthase associated protein 4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGGCACGCGGTGGCGTGAGGGCGGAGT |
| Internal bar code: | ACGAGCGGTCGGACAGTAAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 546 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 603 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATGGTAAACGTGTGGAT |
| Suggested primer 2: | AGAAGCTTGCCACGAACCTA |