| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.219804 |
| Chromosome: | chromosome 2 |
| Location: | 7003795 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g147850 | CLPY2,HSLU2,HUV2 | (1 of 2) K03667 - ATP-dependent HslUV protease ATP-binding subunit HslU (hslU); Peptidase subunit of mitochondrial HslUV protease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAATCCGCCAGCGTGCATAGCCTCCGAC |
| Internal bar code: | GTTTAATGCTTCCGACTTTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 182 |
| LEAP-Seq percent confirming: | 99.3464 |
| LEAP-Seq n confirming: | 152 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCACTGTGCTACACCCCA |
| Suggested primer 2: | CTGCTGCTCAGACGTGAGAC |