| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219804 |
| Chromosome: | chromosome 2 |
| Location: | 7004322 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g147800 | SEC14 | (1 of 13) IPR001251//IPR011074 - CRAL-TRIO lipid binding domain // CRAL/TRIO, N-terminal domain; Putative phosphoglyceride transfer protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGTCCCTGAGCAGGGTCTGGACGACTGG |
| Internal bar code: | CAATCTGCGGGCTTCCGAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 979 |
| LEAP-Seq percent confirming: | 99.587 |
| LEAP-Seq n confirming: | 1688 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATACTGACGCAGGTTGTGG |
| Suggested primer 2: | TGTCGCACATCCGTACATTT |