| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.219826 |
| Chromosome: | chromosome 2 |
| Location: | 6866300 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g119600 | PSD1 | Phosphatidylserine decarboxylase; (1 of 1) 4.1.1.65 - Phosphatidylserine decarboxylase / PS decarboxylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCCTCCCCCCCTCGCCGCGCCTCCCCGC |
| Internal bar code: | GCTTATTTTAGCTTTAATCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 549 |
| LEAP-Seq percent confirming: | 99.7814 |
| LEAP-Seq n confirming: | 2282 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCCGACTTATGCTGCTTG |
| Suggested primer 2: | CTCGCCAGCGTTTCCTATAC |