| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219837 |
| Chromosome: | chromosome_1 |
| Location: | 5793432 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g041400 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCGCGCCCGTGCGACCCTGGGCGCAAAGGT |
| Internal bar code: | GAACGATATGAGATGAAACCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 996 |
| LEAP-Seq percent confirming: | 69.1217 |
| LEAP-Seq n confirming: | 1267 |
| LEAP-Seq n nonconfirming: | 566 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCGATGCACCCAAGTATG |
| Suggested primer 2: | CACCGTTTTTGTGTCAATGC |