Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.219837 |
Chromosome: | chromosome_7 |
Location: | 5019668 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g347050 | RSP7 | Radial spoke protein 7 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCGTGGGAAGGGGGAGGGTTGAAGAGGCT |
Internal bar code: | CTGATTTGGGCATAATGGTAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 599 |
LEAP-Seq percent confirming: | 85.6983 |
LEAP-Seq n confirming: | 3835 |
LEAP-Seq n nonconfirming: | 640 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAAAGCTGGTCTTCCGACTG |
Suggested primer 2: | GATTTGTCCATACCCTCCCC |