| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.219852 |
| Chromosome: | chromosome 1 |
| Location: | 5632561 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g040050 | TPK1 | (1 of 1) K00949 - thiamine pyrophosphokinase (thiN, TPK1, THI80); Thiamine pyrophosphokinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCAGCCACAGAGCAACACGGTGGGAAT |
| Internal bar code: | CTTTCCGATCGGTGGTCAGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 97 |
| LEAP-Seq percent confirming: | 98.8571 |
| LEAP-Seq n confirming: | 173 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGGTAGTGGGGAATCAT |
| Suggested primer 2: | TCAAATACTCACCGCGTGTC |