| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.219885 |
| Chromosome: | chromosome 14 |
| Location: | 356757 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610250 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGACGACCAGCCATTAGACATGTACTCG |
| Internal bar code: | TGGTATAGACCGAAGTAATTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 274 |
| LEAP-Seq percent confirming: | 99.3292 |
| LEAP-Seq n confirming: | 4294 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCTGCCCCTACAACTCAG |
| Suggested primer 2: | ATACCGGTAGCGTCAATTCG |