| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.219928 |
| Chromosome: | chromosome 11 |
| Location: | 640140 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467616 | Dyf-13,IFT56,Ttc26 | (1 of 3) PF12895 - Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3); Intraflagellar Transport Protein 56 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGCCGCGGCCTGTATACAGCGGTGCAGA |
| Internal bar code: | GCGGAGTAAGGTTGCGGTCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 276 |
| LEAP-Seq percent confirming: | 85.9186 |
| LEAP-Seq n confirming: | 4625 |
| LEAP-Seq n nonconfirming: | 758 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGTCATATGTCTGTGGC |
| Suggested primer 2: | ATAGCCATGCTCCCACACTC |