Insertion junction: LMJ.RY0402.219942_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.219942
Chromosome:chromosome 6
Location:7684032
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g301800 MFT19 Major facilitator superfamily transporter; (1 of 2) PF05977 - Transmembrane secretion effector (MFS_3) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCTCGCCACATCCACCACGCATACGTCGC
Internal bar code:CAGACCAGGGAAGACGATGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1092
LEAP-Seq percent confirming:99.5639
LEAP-Seq n confirming:20091
LEAP-Seq n nonconfirming:88
LEAP-Seq n unique pos:96

Suggested primers for confirmation by PCR

Suggested primer 1:CTCACCGCAGATACTGCAAA
Suggested primer 2:CGGTCCTGACACGAAAAACT