| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.219948 |
| Chromosome: | chromosome_13 |
| Location: | 3020708 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g584400 | FAP189 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AAGGAGGAGATCACCGGCAAGGACCTGTAC |
| Internal bar code: | GCGCGGAATTTAACTGGGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 670 |
| LEAP-Seq percent confirming: | 97.6445 |
| LEAP-Seq n confirming: | 456 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAAAGCGGAGGTAAAGGGG |
| Suggested primer 2: | CCTCGTTGATGATGTGGTTG |