| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.219954 |
| Chromosome: | chromosome 12 |
| Location: | 4225509 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g518900 | AKR9 | (1 of 4) 1.1.1.65 - Pyridoxine 4-dehydrogenase / Pyridoxine dehydrogenase; Aldo/keto reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGAGTGCTGGCAGCAGCAGCAACAGCAGC |
| Internal bar code: | TGGTGCGCACGGTCACTGAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 465 |
| LEAP-Seq percent confirming: | 99.5676 |
| LEAP-Seq n confirming: | 1612 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACACGGGTGAGTGGATGAA |
| Suggested primer 2: | GTCTTCACATCCCACGGACT |