| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219975 |
| Chromosome: | chromosome 16 |
| Location: | 4145524 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g669126 | ADH2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAATTCTATGGACTAGGGGGTGCGGGGC |
| Internal bar code: | TAGGTTACTGCTGCTTCGTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1020 |
| LEAP-Seq percent confirming: | 99.6569 |
| LEAP-Seq n confirming: | 15103 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGTGGTTTGCAGCTGTG |
| Suggested primer 2: | AACTATTTCGCCCGTGTGAC |