| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219987 |
| Chromosome: | chromosome 5 |
| Location: | 1884161 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241629 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGAAAGGGCGTGTGTGCCGTTGGGATCC |
| Internal bar code: | GTGAGCCGAGCGGACATGCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 925 |
| LEAP-Seq percent confirming: | 83.4897 |
| LEAP-Seq n confirming: | 445 |
| LEAP-Seq n nonconfirming: | 88 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATACCTTGTCCCAACCAC |
| Suggested primer 2: | AATTGTCCATGAAGCAAGGG |