| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.219992 |
| Chromosome: | chromosome_1 |
| Location: | 695831 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g003650 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCGTTACGGCATGTCTGCTGTAGTAGCTGA |
| Internal bar code: | CCGGTACATCGGATCCGGCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 573 |
| LEAP-Seq percent confirming: | 90.9314 |
| LEAP-Seq n confirming: | 371 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACTGCGGCCTTTCTGACT |
| Suggested primer 2: | CCACACACAGACTCAATGGG |