Insertion junction: LMJ.RY0402.220025_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.220025
Chromosome:chromosome 11
Location:1075514
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467675 (1 of 6) PF12796//PF13920 - Ankyrin repeats (3 copies) (Ank_2) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTGGAGTGTGTCGCGAATGAGCGTGCTA
Internal bar code:GATAGATCCTCAAACATCGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:184
LEAP-Seq percent confirming:98.6248
LEAP-Seq n confirming:1004
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TGGACTACTTGGATCCGTCC
Suggested primer 2:CTAGCGCCACTCGGTATAGG