Insertion junction: LMJ.RY0402.220026_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.220026
Chromosome:chromosome_14
Location:1493764
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre14.g618300 sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):ACAGGGCTCCAGTAGATCTGGCAACCGCAG
Internal bar code:TGCGCCTGCCGAGGGGTGAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:428
LEAP-Seq percent confirming:99.1517
LEAP-Seq n confirming:2104
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGCTGACATGAGCAGTCT
Suggested primer 2:GGCAAAAGTCACTCGCACTT