| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.220051 |
| Chromosome: | chromosome 8 |
| Location: | 1691320 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g365825 | (1 of 1) 1.14.99.42 - Zeaxanthin 7,8-dioxygenase / Zeaxanthin 7,8(7',8')-cleavage dioxygenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGTCGGCAGTTGCCGTCTTACTTGCATT |
| Internal bar code: | TAGTAACGTGGCCTCTCCAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 316 |
| LEAP-Seq percent confirming: | 81.4044 |
| LEAP-Seq n confirming: | 626 |
| LEAP-Seq n nonconfirming: | 143 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTATGGTTGTGGCAGTGG |
| Suggested primer 2: | TGTGGCGCATACATGTTTTT |