| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.220052 |
| Chromosome: | chromosome 16 |
| Location: | 5144019 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683550 | (1 of 2) 1.6.2.4 - NADPH--hemoprotein reductase / TPNH-cytochrome c reductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAGTGGGCGAGGCTGTGCTGTTCTTCGG |
| Internal bar code: | GTTTCTGGCGATGTGTCTATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 218 |
| LEAP-Seq percent confirming: | 99.5585 |
| LEAP-Seq n confirming: | 5186 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCTGTCGCTTCAAGACCA |
| Suggested primer 2: | AATGGAGTTGGAGTGGTTGC |