| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.220070 |
| Chromosome: | chromosome 14 |
| Location: | 691249 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g612633 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAGTCAGGGTGCCCCCGTGAAATAGCCG |
| Internal bar code: | TAACATGGTGTCTTGTCCGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 814 |
| LEAP-Seq percent confirming: | 99.3963 |
| LEAP-Seq n confirming: | 3293 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGTCCTTTACGCAAAAT |
| Suggested primer 2: | GAAGCCACATCACACCACAC |