| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220071 |
| Chromosome: | chromosome 6 |
| Location: | 6458594 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g292800 | UBC17 | E2 Ubiquitin conjugating enzyme; (1 of 2) K06689 - ubiquitin-conjugating enzyme E2 D/E (UBE2D_E, UBC4, UBC5) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATACCCTACTCAGCCCAGCCCTCCACCCC |
| Internal bar code: | GTAGGACCGACGCGTGGTCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 634 |
| LEAP-Seq percent confirming: | 99.5316 |
| LEAP-Seq n confirming: | 425 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCACCCTGTTCTTCGTGGT |
| Suggested primer 2: | CCCTCCTCCCTACCTTTCTG |