| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.220071 |
| Chromosome: | chromosome 11 |
| Location: | 49775 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467528 | CAV4 | (1 of 5) PTHR10037//PTHR10037:SF218 - VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM // SUBFAMILY NOT NAMED; Voltage-gated Ca2+ channel, alpha subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCACACCTGCATTTACGTGTCCTAGCT |
| Internal bar code: | GCGGCTGCCTGTGTGTTTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1072 |
| LEAP-Seq percent confirming: | 96.7655 |
| LEAP-Seq n confirming: | 359 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCGGCATTGGTAGTGTTG |
| Suggested primer 2: | CTGGCCTTTACCATCGTGTT |