| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.220085 |
| Chromosome: | chromosome 12 |
| Location: | 3544490 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513400 | EBG1 | Endo-1%252C3(4)-beta-glucanase; (1 of 1) 3.2.1.6 - Endo-1,3(4)-beta-glucanase / Laminarinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACTCCTGGCGGTACTGGACGCCGCCGTC |
| Internal bar code: | GAAGTCTATGTGCCGCGCGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1073 |
| LEAP-Seq percent confirming: | 99.782 |
| LEAP-Seq n confirming: | 10525 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGCAGCTGTACACGATTG |
| Suggested primer 2: | ATACGTCACGGCCTAAGTGG |