| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220085 |
| Chromosome: | chromosome 14 |
| Location: | 533317 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611484 | (1 of 4) IPR023485 - Phosphotyrosine protein phosphatase I superfamily | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGAGGCCGGCACCGCGGGACAGCTCAGT |
| Internal bar code: | AGGCGTGAGGACTGGTCGCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 27 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGAGACAGAGGTGGCAAG |
| Suggested primer 2: | CTGCGGGTTTATGGTTGACT |