| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.220088 |
| Chromosome: | chromosome 2 |
| Location: | 1821810 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g087050 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCGCAGTGCTGGCCGCCGTCGAAGACAT |
| Internal bar code: | GTTTTACCGATCTCGGGTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 426 |
| LEAP-Seq percent confirming: | 72.1371 |
| LEAP-Seq n confirming: | 989 |
| LEAP-Seq n nonconfirming: | 382 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCACCACAAATCGAAACA |
| Suggested primer 2: | ATGTTATTAGCACCAGGCGG |