| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.220098 |
| Chromosome: | chromosome 1 |
| Location: | 1684881 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g008891 | (1 of 2) 2.8.1.1//2.8.1.2 - Thiosulfate sulfurtransferase / Thiosulfate thiotransferase // 3-mercaptopyruvate sulfurtransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGGTGACAAGGGTCGTAGGAGGGCGAAG |
| Internal bar code: | GACTAAGAGATAGGACGTATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 914 |
| LEAP-Seq percent confirming: | 99.2719 |
| LEAP-Seq n confirming: | 2318 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCGTACAGAAGCAGGCTC |
| Suggested primer 2: | CGTCATCACCACTATCGCAC |