| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220102 |
| Chromosome: | chromosome 8 |
| Location: | 637359 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g359950 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTTGTTCGTCATGGCGATGCCTTAGCTA |
| Internal bar code: | TCAATACGTGGCAAGCCGACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 940 |
| LEAP-Seq percent confirming: | 96.6587 |
| LEAP-Seq n confirming: | 10530 |
| LEAP-Seq n nonconfirming: | 364 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGTGAAGTAGATGCGGT |
| Suggested primer 2: | AACATACTGACGAGGGACCG |