| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220118 |
| Chromosome: | chromosome 9 |
| Location: | 4751913 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397216 | (1 of 3) K01832 - thromboxane-A synthase (TBXAS1, CYP5A) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCAACCGCAGCAGTACGGCGTCATCGGC |
| Internal bar code: | GTCGGAACGCCGACCGAATAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 603 |
| LEAP-Seq percent confirming: | 72.7756 |
| LEAP-Seq n confirming: | 11091 |
| LEAP-Seq n nonconfirming: | 4149 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | GGAAGGATGTCATGCCAAGT |