| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.220145 |
| Chromosome: | chromosome 11 |
| Location: | 2989807 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g478350 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGATCGACCTCTGCGCCGCGACGCAGCTG |
| Internal bar code: | TCGGCCCCCGGGTCCACGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 576 |
| LEAP-Seq percent confirming: | 91.8806 |
| LEAP-Seq n confirming: | 5296 |
| LEAP-Seq n nonconfirming: | 468 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAGTAGTTCCAAGGACCA |
| Suggested primer 2: | GAAGGACGAGGGTAAGGGAC |