Insertion junction: LMJ.RY0402.220150_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.220150
Chromosome:chromosome 17
Location:4199049
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g730150 (1 of 26) IPR029058//IPR029059 - Alpha/Beta hydrolase fold // Alpha/beta hydrolase fold-5 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAACATACGGTTTTGCGTTGTGAACCGCC
Internal bar code:AACACGAGAGTGGATTTAACTT

Confirmation - LEAP-Seq

LEAP-Seq distance:533
LEAP-Seq percent confirming:98.9011
LEAP-Seq n confirming:360
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGACGGTCAAAGCAATTT
Suggested primer 2:ATGGTGACGTAAAAGGCCAG