| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220197 |
| Chromosome: | chromosome 9 |
| Location: | 2825644 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388750 | TEF21 | (1 of 1) PTHR11200:SF170 - PHOSPHOINOSITIDE PHOSPHATASE SAC1; Phosphoinositide phosphatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAGCACGTGTGCACGCCCGCCTGCACCC |
| Internal bar code: | GTTCTAGATGTGTACGCATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1087 |
| LEAP-Seq percent confirming: | 99.7383 |
| LEAP-Seq n confirming: | 2287 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGGATCACAAGACGTGG |
| Suggested primer 2: | GTAACTGTGTGGGCGTTGTG |