| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.220214 |
| Chromosome: | chromosome 11 |
| Location: | 313388 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467563 | (1 of 10) PTHR10174 - RETINALDEHYDE BINDING PROTEIN-RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGATGTCCGTGAATTGGCAATCGTGTTG |
| Internal bar code: | CCCTTCGCTTCGCCTCCAGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 503 |
| LEAP-Seq percent confirming: | 94.2822 |
| LEAP-Seq n confirming: | 2325 |
| LEAP-Seq n nonconfirming: | 141 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCATTGAAGATCAGGTGT |
| Suggested primer 2: | GAGCAACGCCCACATCTATT |