| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.220220 |
| Chromosome: | chromosome 6 |
| Location: | 6497770 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g293100 | (1 of 2) PTHR19957:SF124 - SYNTAXIN-8B-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTTTGGCATAGGCGTTGAAGCAGCGCT |
| Internal bar code: | TTCACGTAAGGCTTGGTCCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 534 |
| LEAP-Seq percent confirming: | 99.9021 |
| LEAP-Seq n confirming: | 8164 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACAATGACACTTCCCGTG |
| Suggested primer 2: | CCTGGGGTAGATAGGCAACA |