| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.220244 |
| Chromosome: | chromosome_4 |
| Location: | 3419693 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g227600 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGGCCGCTGAGAGGGCATACCGTATACGG |
| Internal bar code: | CTTACTGATGGACGCTGGGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 635 |
| LEAP-Seq percent confirming: | 90.21 |
| LEAP-Seq n confirming: | 3050 |
| LEAP-Seq n nonconfirming: | 331 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCTCGTTATTTTCGCTC |
| Suggested primer 2: | GCCGCCATGAACTTGTAGAT |