| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.220250 |
| Chromosome: | chromosome 15 |
| Location: | 1137281 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g641050 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCATCCCGCCCACACCACATCACACACC |
| Internal bar code: | TATTACCTCGTGCCTCTAAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1446 |
| LEAP-Seq percent confirming: | 93.0406 |
| LEAP-Seq n confirming: | 12246 |
| LEAP-Seq n nonconfirming: | 916 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAATGCTACCGTTCGTGCC |
| Suggested primer 2: | ACCTGACCACGTGGAAGAAC |