| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.220264 |
| Chromosome: | chromosome 2 |
| Location: | 1249047 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082550 | ZEP,ZEP1 | Zeaxanthin epoxidase 1; (1 of 1) K09838 - zeaxanthin epoxidase (ZEP, ABA1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTCCTGAGGTCCTGCTAACGACGGGGCG |
| Internal bar code: | GGTAATTGCTGGACAGCGAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 33 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTGCATCTTCACCTTGA |
| Suggested primer 2: | TGACAGGTACGGAAAAAGGG |