| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220330 |
| Chromosome: | chromosome 12 |
| Location: | 2781366 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g502900 | (1 of 3) 3.4.24.13 - IgA-specific metalloendopeptidase / Immunoglobulin A1 proteinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGGGGGACCGCTCAGAGGCATCCGGCG |
| Internal bar code: | AGTATTCCGCAAGCTCGCGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 383 |
| LEAP-Seq percent confirming: | 99.6615 |
| LEAP-Seq n confirming: | 3533 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTGAGATGACGAAGAA |
| Suggested primer 2: | GGGAGCGAGGCATTATACAG |